氧化应激过程的电化学分析

电化学论文

氧化应激过程的电化学分析

田 阳

（同济大学化学系，上海，200092，E-mail：yangtian@mail.tongji.edu.cn， 电话：

021-********， 传真：021-********）

氧化还原反应作为贯穿整个生命迹象的一条主线，始终将生物体的重要生命过程联结在一起。由于内源性和/或外源性刺激致生物体代谢异常而骤然产生大量自由基，当自由基浓度大大超过机体自身抗氧化体系的还原能力时，生物体就处于氧化应激状态。氧化应激过程不仅调节诸多的生理过程和生化反应，同时也造成细胞、亚细胞结构、生物大分子的氧化损伤，因此它是造成癌、心血管疾病、糖尿病、神经系统疾病等的共同病因。然而, 如何对生物体产生的活性氧等自由基进行原位、实时、动态分析，仍然是一项具有挑战性的分析化学课题。

近年来，本课题组致力于活性氧及氧化应激化学过程的电化学分析研究，针对本领域的关键电分析化学问题，进行了探索与研究。(1)基于纳米及分子的巧妙设计，建立了一系列活性氧电化学分析的新方法; (2) 通过光电化学手段，制备了成本低廉、导电性好的生物芯片，构筑了细胞活性氧电化学分析及氧化应激化学过程研究的一体化平台;(3) 在方法学的创新及细胞分析平台建立的基础上，进一步探索了细胞氧化应激过程中活性氧与生理、病理活动之间的关系。

参考文献

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Electroanalytical Chemistry on Oxidative Stress

Yang Tian

(Department of Chemistry, Tongji University, Siping Road 139, Shanghai 200092, P.

R. China, E-mail: yangtian@mail.tongji.edu.cn)

Ageing and many human diseases are associated with oxidative stress, which is a condition reflected by a loss of redox signaling and control resulting in irreversible cellular damage by reactive oxygen/nitrogen species. Despite the wide use of biomarkers of oxidative damage in clinical research, accurate quantification of oxidative stress remains elusive. Our group is focusing on electrochemical study on oxidative stress: (1) A series of electrochemical approaches have been established for determination of reactive oxygen species (ROS), which are related with oxidative stress; (2) Low-cost and high-conductivity protein microarrays have been fabricated for real-time extracellular ROS detection; (3) Based on the developed methods and microarrays, the relationship between ROS and physiological and pathological events has been investigated.

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